性状:称取本品41.5g,加入蒸馏水或去离子水1 L ,搅拌加热煮沸至完全溶解,分装三角瓶,121℃高压灭菌15 ml。冷至50℃左右,每250ml培养基加入过滤除菌的0.12%多粘菌素B硫酸盐水溶液2.5ml和1.2%磺胺嘧啶钠水溶液2.5ml,摇匀。
Suspend 41.5 g in 1 L of distilled or deionized water.Heat with frequent agitation and boil to completely dissolve the powder.Distribute into flask.Autoclave at 121℃ for 15 minutes and then cool to 50℃.Add 2.5 ml of 0.12% sterile polymyxin B and 2.5 ml of 1.2% sterile sulfadiazine to 250 ml of the medium and mix thoroughly.
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