性状 白色结晶或结晶性粉末。水溶液在空气中不吸收二氧化碳，0.1mol/L水溶液pH10.4。对铜、铝有腐蚀作用。溶解度(：550 g/L水 (25°C)，79.1MG/ML 乙二醇，26MG/ML 甲醇 (25°C)，22.0MG/ML 95% 乙醇- 14MG/ML DMF (25°C)，2.0MG/ML 丙酮，0.5MG/ML 乙酸乙酯(25°C)，0.05MG/ML 氯仿，<0.05MG/ML CCL4 (25°C)。
熔点：170℃
沸点：219-220℃(10 mmHg)
质量标准
品级Grade USP/EP,≥99.9% (T),已细胞培养测试
（EP, USP, cell culture tested, ≥99.9% (T))
外观Appearance White Crystalline Powder
灼烧残渣Residue on ignition (Ash) ≤ 0.1 %
水溶性Solubility Colorless，Clear，200 g plus 300 ml of H2O
细胞培养测试Cell Culture Test Pass
DNA酶，外切酶DNAse, Exonuclease Detection None Detected
核酸内切酶NICKase,Endonuclease Detection None Detected
蛋白酶检测Protease Detection None Detected
氯Chloride (Cl) ≤ 100 ppm
内毒素Endotoxin Level ≤ 1 EU/mg
Iron (Fe) ≤ 1 ppm
ICP atomic emission
重金属Heavy Metal(as Lead) ≤ 2 ppm
鉴别试验Identity Test B (EP) Pass
鉴别试验Identity Test C (EP) Pass
鉴别试验Identity Test A (USP) Pass
水分Water (by Karl Fischer) ≤ 0.2 %
干燥失重Loss on Drying ≤ 0.5 %
(EP)
UV absorption 290 nm, 1 % in H2O ≤0.05
UV absorption 260 nm, 10 % in H2O ≤0.03
UV absorption 280 nm, 10 % in H2O ≤0.02
UV absorption 430 nm, 10 % in H2O ≤0.004
UV absorption 290 nm, 40 % in H2O ≤0.2
项目 ACS级
(ACS)
外观Appearance 白色结晶粉末或针状结晶
红外光谱鉴别Infrared spectrometry 符合
含量Assay (以干基计) 99.8 - 100.1 % (HCl-titration)
水分Water <2 % (K.F.)
重金属Heavy metals (as Pb) <5 ppm
吸光度Absorbance passes test
不溶性物质Insoluble matter <0.005 %
铁Fe < 5 ppm
品级Grade 分子生物学级
(grade for molecular biology, ≥99.9% (T))
含量assay ≥99.9% (T)
灼烧残渣Residue on ignition ≤0.01% (as SO4)
干燥损失loss on drying ≤0.5% ， 110 °C
pH 10.5-12.0 (25 °C, 4 M in H2O)
useful pH range 7 - 9
pKa (25 °C) 8.1
水溶性solubility H2O: soluble4 M at 20 °C, clear, colorless
氯chloride (Cl-): ≤20 ppm
硫酸根sulfate (SO42-): ≤5 ppm
Al: ≤5 ppm
As: ≤0.1 ppm
Ba: ≤5 ppm
Bi: ≤5 ppm
Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Li: ≤5 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Mo: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Sr: ≤5 ppm
Zn: ≤5 ppm
λ 4 M in H2O
UV absorption
λ: 260 nm A: ≤0.10
λ: 280 nm A: ≤0.08
DNA酶DNases, none detected
RND酶RNases, none detected
不溶物insoluble matter, passes filter test
磷酸酶phosphatases, none detected
蛋白酶proteases, none detected
品级Grade 细胞培养级
(for cell culture , ≥99.9% (T))
外观Appearance 白色粉末
纯度Purity ≥99.9% (T)
水溶解试验solubility in water clear colorless , 200g plus 300ml of water
水分water ≤0.2%(K.F)
紫外吸收UV absorbance ≤ 0.05 at 290nm (40%w/w)
重金属heavy metals (as lead) ≤2 ppm
细胞培养测试Cell culture test pass
贮存 密封保存。
用途 生物缓冲剂，缓冲范围pH7.0-9.0。酸性气体吸收剂。酸量滴定法基准物质。电流滴定法测定蛋白质中的巯基。纸上电泳分离血清蛋白。淀粉平板电泳分离毒素。
联合国编号
Risk： R36/37/38 -
Safety : S26- S36-
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